FIG. 8.

(a) Time course of EGFP-expressing HeLaEBNA cells after cotransduction with HC-AdFLPe (20 MOI) and the indicated substrate vectors (20 MOI each). Cells transduced with HC-AdSVEBNAs remained EGFP positive for longer time periods than did cells transduced with HC-AdSVEBNAsΔFR, which carried a truncated oriP. (b) The corresponding Southern blot membrane. The circular genomes of HC-AdSVEBNAs and HC-AdSVEBNAw are represented by signals at 2,200 bp; their linear counterparts are represented by signals at 3,300 bp. HC-AdHygEBNA was not included in the flow cytometry experiments due to weak EGFP-specific fluorescence.