FIG. 9.

(a) PCR assay for detection of circular vector genomes that have been generated by joining the vector ITRs. The indicated primers bigcircseq and bigcircrev can only form a product when there are appropriate joint circular vector genomes. The expected PCR product is 974 bp. (b) PCR with genomic DNA from HeLaEBNA1 cells transduced with the indicated substrate vectors (100 MOI each). Genomic DNA was prepared at different time points posttransduction (numbers above lanes). Ctr, genomic DNA from untransduced control cells; H, water control; P, pFrt#39 (which was used as a plasmid control and should result in a 3,900-bp product).