Table 2.
Comparative proteomic analysis: proteins up-regulated by Cardiogenol C in HBPCs
| Proteins identified | Observed MW(kDa)/pI | Theoretical MW (kDa)/pI | Accession number | Folds increase | Reported functions |
|---|---|---|---|---|---|
| COP9 signalosome complex subunit 6 | 41/5.4 | 35.9/5.5 | O88545 | 1.7 | A complex involved in various cellular and developmental processes. It is an essential regulator of the ubiquitin conjugation pathway |
| Emerin | 27/4.6 | 29.4/4.9 | O08579 | 2.3 | Emerin may be required for the stability and normal function of rigorously moving nuclei in skeletal muscle and heart |
| Methylenetetrahydro folate reductase (Mthfr) | 70/4.9 | 74.7/5.2 | Q9WU20 | 2.4 | Folate is important for normal heart development. It is a key enzyme in folate and homocysteine metabolism |
| Myosin light polypeptide 6 | 14/4.7 | 16.8/4.6 | Q60605 | 1.4 | Regulatory light chain of myosin |
| Myosin light polypeptide 3 | 19/4.9 | 21.8/5.0 | P08590 | 1.6 | Regulatory light chain of myosin |
| Procollagen-lysine,2- oxoglutarate 5- dioxygenase 2 precursor (Plod2) | 80/6.5 | 84.5/6.3 | Q9R0B9 | 4.0 | Essential for the stability of the intermolecular collagen cross-links; highly expressed in heart, lung, kidney, eye, ovary, and placenta |
| Protein C-ets-1 | 55/4.8 | 50.2/5.0 | P27577 | 1.7 | Transcription factor. Binds to DAXX. Interacts with UBE2I. Essential for normal coronary and myocardial development in chicken embryos |
| Salt-inducible kinase (SIK1) | 81/6.4 | 85.0/6.4 | Q60670 | 3.8 | Transient role during the earliest stages of myocardial cell differentiation and/or primitive chamber formation and may also be important for the earliest stages of skeletal muscle growth and/or differentiation. Potential role in G2/M cell cycle regulation |
| SWI/SNF related protein Smarce1 | 49/4.5 | 46.6/4.9 | O54941 | 1.9 | Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology) |
| Transcription cofactor HES-6 | 23/5.3 | 24.4/5.2 | Q9JHE6 | 1.4 | Up-regulate the transcription of ASCL1 and TCF3. Promotes cell differentiation |
| Tripartite motif- containing protein 54 | 36/5. | 40.3/5.1 | Q9BYV2 | 1.6 | It may bind and stabilize microtubule during myotube formation |
| Troponin C | 17/4.2 | 18.4/4.0 | P19123 | 1.4 | Troponin is the central regulatory protein of striated muscle contraction. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments |