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The purified Saccharomyces castellii Rap1 DNA-binding domain (scasRap1-DBD) binds as efficiently to telomeric double-stranded DNA as the full-length protein. EMSA of increasing amounts of the full-length scasRap1 (lanes 2-4) and scasRap1-DBD (lanes 5-7) incubated with the labeled double-stranded 19-mer telomeric DNA probe (Scast19F). No protein was added in lane 1. Protein was added in 2-fold increasing amounts.
