Table 1.
Oligonucleotides used in this study
| Name | Sequence 5' to 3' | Length (nt) |
|---|---|---|
| Scast10C | GGGTGTCTGG | 10 |
| Scast11C | GGGTGTCTGGG | 11 |
| Scast12C | GGGTGTCTGGGT | 12 |
| Scast12F | GGTGTCTGGGTG | 12 |
| Scast14C | GGGTGTCTGGGTGT | 14 |
| Scast16F | GGTGTCTGGGTGTCTG | 16 |
| Scast19F | GTGTCTGGGTGTCTGGGTG | 19 |
| Scast14C1 | CGGTGTCTGGGTGT | 14 |
| Scast14C2 | GCGTGTCTGGGTGT | 14 |
| Scast14C3 | GGCTCTCTGGGTGT | 14 |
| Scast14C4 | GGGAGTCTGGGTCT | 14 |
| Scast14C5 | GGGTCTCTGGGTGT | 14 |
| Scast14C6 | GGGTGACTGGGTGT | 14 |
| Scast14C7 | GGGTCTGTGGGTGT | 14 |
| Scast14C8 | GGGTGTCAGGGTCT | 14 |
| Scast14C9 | GGGTGTCTCGGTGT | 14 |
| Scast14C10 | GGGTGTCTGCGTGT | 14 |
| Scast14C11 | GGGTCTCTGGCTGT | 14 |
| Scast14C12 | GGGTGTCTGGGACT | 14 |
| Scast14C13 | GGGTGTCTGGGTCT | 14 |
| Scast14C14 | GGGTGTCTGGGTGA | 14 |
Scast12C represents the minimal binding site (MBS). Bold letters in Scast14C1-Scast14C14 indicate mutated positions.