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Validation of microarray data by quantitative real-time. RT-qPCR fold-changes are shown for five genes upregulated in all microarray pairwise comparisons (Rub/Sun, Pool R/Sun, and Pool R/Pool S) 48 hours after P. parasitica inoculation, and compared with fold-changes obtained by microarray analysis. RT-qPCR data were normalized to the two most stable endogenous control genes (UBQ and CYP).
