Table 1.
Oligonucleotide primers used for RT-qPCR analysis.
| Description | CitEST | Forward/Reverse | Amp | |
|---|---|---|---|---|
| Selected genes to validate the microarray | ||||
| LEA | Lea 5 | CAS-PT-303903 | TCGGACTGGTATCATGGA/GTAGTACCCAGTGATGGGA | 99 |
| MIR | miraculin | CAS-CR-215276 | AGCCCTGTAATGAAGAACC/TAGCAACGTTTCAGCTCC | 100 |
| TIR | TIR-NBS-LRR | CAS-CS-103511 | CATGATGAGGACGTGGG/AAGTGATCCGACTCGAC | 103 |
| RPS4 | Disease resistance RPS4 | CAS-PT-300852 | CCAAGATCTTGAATATCTTCCC/GCAAGTTGAGCTCAATTAGG | 112 |
| UNC | unclassified proteins | CAS-PT-310250 | TCTCTTGTTCTTCATGCAGT/TATGCATCTTGCCTTCATTC | 116 |
| Selected endogenous control genes | ||||
| ETEF2 | eukaryotic translation elongation factor 2 | CAS-PT-306679 | TTGAGGCTTCTGAATCGAG/CTTTCCAGATGAACCTCTCC | 97 |
| EGIDH | NADP-isocitrate dehydrogenase | CAS-CS-112964 | CATTGAACATGCAGTTGAGG/ATTCTCATGACGTGTCGG | 91 |
| CYC | cyclophilin | CAS-PT-301486 | AGAGTATGCAGAGGAATGG/GTCCTTAACAGAAGTCCGT | 107 |
| UBQ | ubiquitin | CAS-PT-300961 | TTCGTCAGTTGACTAATCCT/GTTGCTGTGTTGACTGTG | 95 |
| TUB | tubulin | - | TTTGTAAGATCCCTCCGA/TCACCCTCCTGAACATTT | 87 |