Table 1.
Summary of Illumina expressed short reads production and filtration
| Catfish species | No. of tissues | No. of fish | Sequencer | Sequence length* | Reads (X106) | Bases sequenced (X109) | Reads after trimming (X106) | Bases after trimming (X109) |
|---|---|---|---|---|---|---|---|---|
| Channel | 11 | 47 | Illumina GA-II | 36 bp | 48.6 | 1.8 | 47.2 | 1.7 |
| HiSeq 2000 | 100 bp | 173.9 | 17.4 | 171.6 | 13.9 | |||
| Blue | 11 | 19 | Illumina GA-II | 36 bp | 66.9 | 2.3 | 62.1 | 2.2 |
| HiSeq 2000 | 100 bp | 216.6 | 21.7 | 212.5 | 17.4 | |||
| Total | - | - | - | - | 506.0 | 43.2 | 493.4 | 35.2 |
Eleven tissues were used for RNA preparation including brain, gill, head kidney, intestine, liver, muscle, skin, spleen, stomach, heart, and trunk kidney. *Paired-end reads were generated in different lengths of either 36 bp or 100 bp as a result of different sequencers, Illumina GA-II or HiSeq 2000.