Table 5.

Summary of BLASTX searches to annotated protein databases

Assembly	Contigs hit Uniprot	% contigs with hits	Unique protein hits	Contigs hit zebrafish Refseq	% contigs with hits	Unique zebrafish Refseq hits
Reference	32,350	30.9%	17,766	36,597	34.9%	14,874
De novo	24,168	5.7%	12,331	29,688	7.1%	10,781
Total	56,518	10.7%	24,440	66,285	12.6%	19,899

Contigs of two assemblies, the reference assembly with 104,870 contigs and the de novo assembly with 421,229 contigs, were used to search the Uniprot database and the zebrafish Refseq protein database to assess the number of related genes represented by catfish expressed sequences.