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Flow cytometry detection of surface molecules on exosomes from saliva, plasma and breast milk. Exosomes from saliva, plasma and breast milk captured on anti-MHC class II beads were immunostained by using monoclonal antibodies against the tetraspanins CD9, CD63 and CD81 and analysed by flow cytometry. The antibodies (open peaks) were compared with their appropriate isotype controls (filled peaks).
