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. 2011 Jan 19;6:7. doi: 10.1186/1748-717X-6-7

Figure 5.

Figure 5

Influence of BO-1051 on the repair of radiation-induced DSBs. GBM-3 cells growing on slides in 35-mm dishes were exposed to 200 nM of BO-1051 for 24 h, irradiated (2Gy), and then fixed at the specified times for immunofluorescent analysis of nuclear γ-H2AX foci using a confocal microscope. (A) Immunofluorescent microscopy images of GBM-3 cells untreated or treated with 200 nM BO-1051 24 h before irradiation, fixed after 0, 1, 6, 24 h and then stained for γ-H2AX foci. (B) Quantitative analysis of γ-H2AX foci presented in irradiated cells following the above treatments. Filled columns: data from vehicle-treated cells; open columns: data from cells exposed to BO-1051. Values are the means ± SD of 3 independent experiments. * p < 0.05. (C) Distribution of γ-H2AX foci numbers per cell for one representative experiment at 24 h after irradiation. Ctrl: no treatment; IR: ironing radiation at 2 Gy; BO: cells exposed to 200 nM BO-1051; BO/IR: cells exposed to 200 nM BO-1051 for 24 h and then irradiated with 2 Gy of γ-rays. Foci were evaluated in 100 nuclei per treatment for each cell type. Values are the means at least of 3 independent experiments.