Figure 6.

The effects of BO-1051 on radiation-induced tumor growth delay and prolongation of TTF (time to treatment failure) in nude mice bearing GBM-3 xenografts. When tumors reached 150 mm³, the nude mice with established GBM-3 flank xenografts were randomized into 4 groups: control (black circle), radiation (white circle), BO-1051 (black triangle) or BO-1051 plus radiation (white triangle). BO-1051 (50 mg/kg) was delivered via i.p. injection on days 0, 3 and 6, where day 0 begins on the day of randomization. Radiation (4 Gy) was delivered 24 h after the first injection of BO-1051 (day 1 after randomization), which corresponded to the same tumor size. Each group contained at least 8 mice. (A) Tumor growth rates for each treatment group were plotted as the mean relative tumor volume ± SD. Arrows indicate the time of BO-1051 and irradiation treatment. (B) Kaplan-Meier survival rates of nude mice with GBM-3 flank xenografts for each of the four treatments is depicted. Survival analysis was monitored daily. Treatment failure was defined as tumor size greater than 1500 mm³ or the development of severe necrosis requiring euthanasia.