Figure 5.
NAA gain-of-function in pseudoviral particle release. A, luciferase activity of pseudovirions generated in the presence of NAA and NAA mutants containing single or combined substitutions V119E, Y234N, R241M and R345G (N2 numbering). Experiments were performed in triplicates and repeated several times, and error bars indicate standard deviations. B, hemagglutination activity of NAA substitution panel. C, neuraminidase activity of NAC (Av 226-301) substitution mutants measured by the release of fluorogenic substrate 4-MUNANA. Experiments were performed in triplicates and repeated several times, and error bars indicate standard deviations.