Figure 6.
Effect of E119V, N234Y, M241R and G345R single substitutions on NAH function. A, luciferase activity of pseudovirions generated in the presence of NAH single substitution mutants. Experiments were performed in triplicates and repeated several times, and error bars indicate standard deviations. B, hemagglutination activity of NAH substitution panel. C, neuraminidase activity of NAH substitution mutants measured by the release of fluorogenic substrate 4-MUNANA. Values are presented as the fold-change of triplicate samples. Experiments were performed in triplicates and repeated several times, and error bars indicate standard deviations. D, western blot of NA expression in 293T cells and incorporation onto HIV particles.