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Immune reactivities of infected cell proteins and death factors at 12 hpi (A and B) or 15 hpi (C) from vBSΔ27-infected HEp-2 cells plus actinomycin D. Whole-cell extracts were prepared from mock-infected cells or cells infected with HSV-1(vBSΔ27) (Δ27) in the absence or presence of the transcription inhibitor actinomycin D or DMSO solvent as described in Materials and Methods. Immunoblot analyses utilized anti-ICP4, -PARP, and -caspase-3 antibodies.
