FIG. 8.

Immune reactivities of infected cell proteins (A) and death factors (B) and cell morphologies (C) of infected HEp-2 cells. Whole-cell extracts were prepared at 24 hpi from mock-infected cells or cells infected with HSV-1(KOS1.1) or HSV-1(V422) in the absence (−) or presence (+) of CHX as described in Materials and Methods. Immunoblot analyses utilized anti-ICP4, -gC, and -ICP27 antibodies (A) or -PARP and -caspase-3 antibodies (B). The locations of prestained molecular mass markers are indicated in the right margins. 116 and 85, full-length and processed PARP, respectively. (C) Phase-contrast, fluorescence (Hoechst), and merged (Overlay) images of corresponding infected HEp-2 cells. Mock-, HSV-1(KOS1.1)-, and HSV-1(V422)-infected HEp-2 cells in the absence (Without CHX) and presence (CHX Addition) of CHX were visualized at 24 hpi as described in Materials and Methods. Numbers in panels (white) refer to the percentage of cells showing apoptotic condensed chromatin. Magnification, ×40. These cells were used to prepare the whole-cell extracts shown in panels A and B.