Table 2. Oligonucleotide primers.

Name of primer	Nucleotide sequence (5′-3′)	Amplified fragment size	Target genes in reference strain	GBS reference strain
lmb130	GTTGTGAGTTTAGTAATGATAGC	840 bp	lmb	R268 [43]
lmb970	GATATGTCTTGTTCCGCTTG
rogB59	GCTATGATTACTACCCTTCCATTACTC	130 bp	rogB	6313 [23]
rogB189	TTCGATATTCAGAGAGAGTTGACTG
rogB298	GATTCAGGCAGGTTCCCTTT	891 bp
rogB1189	CGGCTATTTGTATCGGAGGA
rogB350	GTGCAACTGCTTATCGCATAC	794 bp
rogB1144	GGTGAGCACAAAGGAGAAGAA
rogB822	TTGGTCTGAGAAGCGTATCG	131 bp
rogB953	GCAACTTTTACCAACTCGTCA
rgfB1	TCTATGGCAAAATGCTTAACG	1086	rgfBD	O90R [25]
rgfD155	TCTCTAAACGTGCCTGTTGAA
rgfD89	ACGAGGAGACGAAAGTGAAT	2277	rgfDAC
rgfC	CGCAAAGTTCTATGGTTCAAAA
rovS114	CAAGGTTTGAGAGAGGAGAGTCA	631 bp	rovS	NEM 316 [24]
rovS745	TCCTGAAGAAGTATCACCAAGTTTT
rovS82	AGCAGATGAGCACCTATCCA	146 bp
rovS228	TGAGTGTGCGCCTTAGAATG
fbsA282	CAACTTATAGGGAAAAATCCAC	123 bp	fbsA	176H4A [20]
fbsA 405	AGTTAACATCGGTCTATTAGC
fbsA86	ATCAAGTCCTGTATCTGCTAT	469 bp
fbsA555rc	TTCATTGCGTCTCAAACCG
fbsB354	GCGATTGTGAATAGAATGAGTG	129 bp	fbsB	NEM316 [18], [45]
fbsB483	ACAGAAGCGGCGATTTCATT
fbsB143	TCGGTCATAAAATAGCGTATGG	1567 bp
fbsB1710rc	AAGAATTCAACGGTCGGCTTCGT
gyrA	CGGGACACGTACAGGCTACT	128 bp	gyrA	NEM316 [45]
rgyrA	CGATACGAGAAGCTCCCACA