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. 2011 Feb 4;6(2):e16770. doi: 10.1371/journal.pone.0016770

Figure 5. Absence of β-galactosidase (β-gal) signal from the thick ascending limbs.

Figure 5

Tubules stained positive for β-gal (green) and Tamm-Horsfall protein (THP, red) were mutually exclusive in kidneys of 2-week-old (A) and 8-week-old (B) mice. Original magnification was 200×. No specific signal was detected on sections incubated with non-immune IgGs in place of primary antibodies (data not shown). C: cortex; M: medulla.