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. 2011 Feb 4;6(2):e16770. doi: 10.1371/journal.pone.0016770

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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In kidneys of 2-week-old (A) and 8-week-old (B) mice, tubules with intense CD28K signal (arrow) did not stain positive for β-gal protein. Some tubules with intermittent CD28K, likely connecting tubules or cortical collecting ducts, demonstrated positive β-gal signal (double arrow). Original magnification was 400×. No specific signal was detected on sections incubated with non-immune IgGs in place of primary antibodies (data not shown).