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. 2011 Feb 4;6(2):e16770. doi: 10.1371/journal.pone.0016770

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Ai. In kidneys of 2-week-old mice, immunostaining pattern of β-gal (green) closely resembled that of aquaporin 2 (AQP2, red). Right panel showed co-localization of the two signals to the same tubules. Original magnification was 100×. Aii. Merged images: Principal cells in the cortex, outer medulla and inner medulla appeared yellow-orange when AQP2 signal co-localized with β-gal signal. Original magnification was 400×. B. β-gal (green) signal was also observed in principal cells that stained positive for AQP2 (red) in the collecting ducts in cortex, outer medulla and inner medulla of 8-week-old mice. Asterisk indicates non-specific staining, which was also observed on sections incubated with non-immune IgGs in place of primary antibodies (data not shown). Original magnification was 400×.