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. 2011 Feb 4;6(2):e16770. doi: 10.1371/journal.pone.0016770

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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β-gal signal (green) was detected in intercalated cells of collecting ducts (arrow) that stained positive for vacuolar H⁺-ATPase B1 (V-ATPase, red) in kidney cortex (Ai) and medulla (Aii) of 2-week-old mice. In 8-week-old mice, β-gal signal (green) was less apparent in the cortex but was noted in the medulla (arrow) (B). Original magnification was 400×. No specific signal was detected on sections incubated with non-immune IgGs in place of primary antibodies (data not shown).