FIG. 3.

Dynamics of PrPres accumulation in MovS6 cells following exposure to sheep prion. PK-treated cell lysates (500 μg of protein) from cultures at the indicated passage p.i. (p1 to p5) were analyzed by Western blotting with 2D6 antibody. MovS6 and MS^0/0 cultures (12th subpassage) were incubated for 4 days with 2.5% brain homogenate prepared from scrapie-diseased (+) or control (−) tgOv mice. MOI, ∼20 ID₅₀ U/cell.