TABLE 1.
Ovine PrP transgenec | Cell clone
|
PrPres detection at p.i. passage no.b:
|
|||
---|---|---|---|---|---|
Designation | Morphology | 1 | 2 | 3 | |
+ | 2D2 (MovS2) | Fusiform | + | + | + |
+ | 2H12 | Fusiform | − | − | + |
+ | 2G8 | Bipolar, then fusiform | − | + | + |
+ | 2A4 | Large, pavimentous | − | − | − |
+ | 6H5 (MovS6) | Bipolar, spindle shaped | + | + | + |
+ | 6A5 | Bipolar, spindle shaped | − | + | + |
+ | 6B4 | Bipolar, spindle shaped | − | − | + |
− | 9G9 (MS0/0) | Bipolar, spindle shaped | − | − | − |
− | 9F10 | Bipolar, then fusiform | − | − | − |
Primary glial cell-enriched cultures established from three donors, designated 2, 6, and 9 (SV40 Tag+/− and mouse PrP0/0 background).
Subpassaging at a weekly 1:10 split ratio. +, PrPres detected; −, PrPres not detected.
Presence (+) or absence (−) of PrP transgene.