Western blot analysis of ATP synthase gamma chain and elongation factor 1-alpha 1 proteins in control and histatin-treated mitochondrial samples. Aliquots of 3 μg of mitochondrial proteins were subjected to 12% SDS-PAGE and Western Blotting using specific antibodies against A, the subunit of ATP synthase (AtpC), B, elongation factor 1 alpha 1 (EEF1A1), or C, cytochrome c (Cytc). Representative Western blots are shown as well as the densitometric analyses of experiments conducted at least twice. Data are represented as the mean ± confidence interval (CI) and statistical evaluation for each experimental condition were determined versus the control sample. *Experimental value significantly different from the control (p<0.05).