FIG. 2.

Sodium orthovanadate does not inhibit JCV infection of glial cells. (A) SVG-A cells grown on coverslips were pretreated with 50 or 100 μM sodium orthovanadate for 1 h at 37°C. Cells were then infected with JCV or SV40 in the continued presence of NaVO₃ for 4 h at 37°C, and anti-JCV or anti-SV40 neutralizing antibodies were added at 4 h postinfection to neutralize any remaining extracellular virus. At 72 h postinfection the cells were washed and fixed, and infection was scored by an indirect immunofluorescence assay for the late viral protein VP1. (B) VP1-positive cells were scored by counting, and the results are graphed.