FIG. 5.

Dominant-negative eps15 constructs inhibit JCV infection. SVG-A cells grown on coverslips were transfected with the indicated GFP- or FLAG-tagged dominant-negative eps15 constructs or control constructs for 24 h. Cells were then infected with JCV, and at 72 h postinfection the cells were fixed and stained with an anti-V antigen (VP1) monoclonal antibody (red). The GFP-expressing constructs were directly visualized, and the FLAG-tagged constructs were visualized using anti-FLAG antibody and an Alexa-Fluor 488-labeled secondary antibody. JCV was unable to infect any of the cells expressing a dominant-negative construct (EH95, D3, and eps15-Y850F). In contrast, JCV readily infected cells expressing control constructs (D3D2 and eps15wt).