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. 2011 Jan 7;30(3):456–467. doi: 10.1038/emboj.2010.348

Figure 2.

Figure 2

Replacement of the GAB and FAB in hVASP with the corresponding DdVASP motifs markedly accelerates VASP-mediated filament elongation. (A) Scheme of hVASP chimeras bearing different domains of DdVASP. DdVASP is shown in colour and hVASP is shown in greyscale. G, GAB; F, FAB; T, tetramerization domain. (B) TIRFM micrographs of the assembly of 1.3 μM actin (30% OG labelled) in TIRF buffer containing 500 nM of the chimera are indicated. Time is indicated in seconds, scale=10 μm. (C) Elongation rates of the chimeras in solution in a concentration rage from 25 nM to 1 μM. (D) (Left) TIRFM micrographs of the assembly of 1.3 μM actin (30% OG labelled) in TIRF buffer in the presence of 200 nM CP on beads saturated with the hVASP chimeras are indicated. Time is shown in seconds, scale=10 μm. (Right) Plots of the lengths of individual filaments versus time yield filament elongation rates. Elongation rates are presented as mean values±s.e.m.