FIG. 4.

BZLF1 decreases TNF-R1 protein and RNA levels. (A) hTERT-HFK, HeLa, and A549 cells were mock infected or infected with adenovirus (MOI of 50) expressing either LacZ or BZLF1 for 48 h. Immunoblot analysis was performed with anti-TNF-R1 and anti-β-actin antibodies. (B) A549 and HeLa cells were transfected with a vector control or a BZLF1 expression plasmid. At 48 h posttransfection, cell lysates were examined by immunoblot analysis with anti-TNF-R1, anti-BZLF1, and anti-β-actin antibodies. HTERT-HFK (C) and A549 (D) cells were mock infected or infected with adenovirus (MOI of 50) expressing either LacZ or BZLF1. Semiquantitative reverse transcription-PCR analysis was performed by using serial dilutions of input cDNA with oligonucleotide primers specific for TNF-R1 mRNA sequences or with primers for β₂-microglobulin sequences.