FIG. 3.

Dependence of A17 phosphorylation on A30 and G7 was not circumvented by a phosphatase inhibitor. BS-C-1 cells were either mock infected (Un) or infected with vT7lacOI or vA30Li in the presence (+) or absence (−) of 50 μM IPTG. Four hours after infection, 100 μM Na₃VO₄ was added to the infected cells as indicated. Ten hours after infection, the cells were harvested and whole-cell lysates were analyzed by electrophoresis in an SDS-4 to 20% gradient polyacrylamide gel in Tris-glycine buffer followed by Western blotting using anti-pTyr or anti-A17N antibody, as indicated on the right. The migration positions and molecular masses of marker proteins are indicated on the left.