FIG. 7.

F10-dependent phosphorylation of A17 was stimulated by A30 and G7 in the absence of other viral late proteins. BS-C-1 cells were infected with vTF7-3 in the presence (+) or absence (−) of AraC and either not transfected (−) or transfected (+) with combinations of plasmids containing the F10V5, G7L, A30L, or A17L ORF regulated by a bacteriophage T7 promoter. Twenty-four hours after infection, the cells were harvested and whole-cell lysates were analyzed by electrophoresis in an SDS-10 to 20% polyacrylamide gel in Tricine buffer followed by Western blotting with anti-pTyr or anti-pThr antibodies. The bands corresponding to the A17 and F10V5 proteins recognized by the anti-pTyr (A17PY) and anti-pThr (A17PT and F10V5PT) antibodies are indicated on the right. The membranes were stripped and reprobed with antibodies against F10V5, G7, A17, and A30, as indicated on the right. The migration positions and molecular masses of marker proteins, in kilodaltons, are indicated on the left.