FIG. 7.
Viral particles produced by HCMV mutants contain equivalent levels of gB. HCMV mutants expressing either gBWT, gBAla900, or gBAsp900 were used to infect HFF at an MOI of 1. Virions, NIEPs, and DBs were purified from the supernatants of infected cells by glycerol-tartrate centrifugation. (A) Virion proteins were visualized by Western blot with a polyclonal antibody raised against whole virus, and gB was visualized with an MAb against gB. For reference, several viral structural proteins are indicated (3). (B) Relative levels of gB compared to total virion proteins quantitated by densitometry of multiple lanes after SDS-PAGE. The values shown are the average of at least three lanes.