TABLE 3.
Transformation of ΔtfoXVF and tfoYVF∷Tn5 strains*
| Recipient strain | Plasmid | Mean transformation frequency† | Range |
|---|---|---|---|
| ES114 (wild type) | pVSV104 (vector) | 54 | 2 to 120 |
| pMulTfoX | 12,000 | 7,700 to 16,000 | |
| pMulTfoY | <0.1‡ | - | |
| AGP200 (ΔtfoXVF) | pVSV104 (vector) | <0.1‡ | - |
| pMulTfoX | 4,400 | 190 to 8,600 | |
| pMulTfoY | <0.1‡ | - | |
| NL1 (tfoYVF∷Tn5) | pVSV104 (vector) | <0.1‡ | - |
| pMulTfoX | 21,000 | 350 to 41,000 | |
| pMulTfoY | 1 | 0.1 to 2 |
*
Grown in minimal media with chitohexaose (GlcNAc)6
†
Average of two experiments (× 109)
‡
Below detection limit, 1 × 10-10