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Influence of the form of extracellular DNA on transformation frequency*
| DNA form | Estimated extent of flanking homology (bp) | Mean transformation frequency† | Range |
|---|---|---|---|
| chromosomal | >104 | 23,000 | 12,000 to 33,000 |
| plasmid | 103 | 22,000 | 16,000 to 28,000 |
| PCR product | 102 to 103 | 530 | 460 to 610 |
V. fischeri strain ES114 carrying pMulTfoX was grown in minimal media (MM) with (GlcNAc)6.
Transformation frequency when equal weights of DNA were added; average of two experiments (× 109).
