Fig. 4.

Number of Tyrosine hydroxylase (TH) immunostained nuclei in the locus coerleus areas of the experimental groups. The AM group was daily treated with the GR extract (400 mg/kg, p.o.) for 2 weeks, and other groups were given sterile saline. Immobilization began 30 min after the treatments. The results of TH-reactivity were analyzed by performing separate one-way ANOVA of neurons among the groups were followed by LSD test. Each value represents the mean±S.E.M. ^*p<0.05 compared to normal group and ⁺⁺p<0.01 compared to control group. (B) Photographs showing the distribution of TH-immunoreactive cells in LC of Normal group (A), Control group (B), GR group (C). Sections were cut coronally at 30 µm and the scale bar represents 200 µm (200×200).