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. Author manuscript; available in PMC: 2011 Feb 6.
Published in final edited form as: Evol Dev. 2009 Sep–Oct;11(5):560–573. doi: 10.1111/j.1525-142X.2009.00362.x

Fig. 3.

Fig. 3

Vasa mRNA and protein is restricted to the small micromere lineage and larval coelomic pouches in sea urchins. (A) In Lytechinus variegatus, vasa mRNA is enriched at the vegetal plate of the blastula, in the gut of the gastrula, and finally in the small micromere lineage of the prism and pluteus larva. (B) In L. variegatus, Vasa protein is enriched in the micromeres of the 16-cell embryo and in the small micromeres of the blastula. (C) In Strongylocentrotus droebachiensis, Vasa protein accumulates in the small micromeres upon their formation, remains enriched in this lineage throughout embryogenesis, and is then enriched in the coelomic pouches of the pluteus larva. (D) In Arbacia punctulata, Vasa protein is enriched in the coelomic pouches of the pluteus larva. (B–D) Vasa protein (red), Hoechst (blue); Vasa immunofluorescence was performed with an antibody raised against Sp-Vasa (Voronina et al. 2008). Scale bars are 20 μm.