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. Author manuscript; available in PMC: 2011 Feb 6.
Published in final edited form as: Evol Dev. 2009 Sep–Oct;11(5):560–573. doi: 10.1111/j.1525-142X.2009.00362.x

Fig. 5.

Fig. 5

Vasa mRNA and protein are enriched in the coelomic pouches of the pencil urchin, Eucidaris tribuloides. (A) Vasa mRNA is enriched at the vegetal plate of the blastula, in the gut of the gastrula, and finally in the coelomic pouches of the larva. To visualize vasa mRNA in the coelomic pouches the time of exposure to the alkaline phosphatase reaction conditions was increased from approximately 6 to 22 h. (B) Vasa protein accumulates uniformly through embryogenesis and is then enriched in the coelomic pouches of the larva. Negative control excludes the primary antibody. Vasa protein (red), Hoechst (blue); Vasa immunofluorescence was performed with an antibody raised against Sp-vasa (Voronina et al. 2008). Scale bars are 20 μm.