Table 3.
Effects of NarX Q412R and V413M alterations on NarL- and NarP-dependent induction.
| Alleleb | β-Galactosidase sp acta |
|||||
|---|---|---|---|---|---|---|
| NarL-dependent induction byc |
NarP-dependent induction byd |
|||||
| None | Nitrate | Nitrite | None | Nitrate | Nitrite | |
| pHG165 | 11 | 10 | 17 | 220 | 230 | 120 |
| narX‡ | 70 | 1800 | 120 | 1760 | 3340 | 3400 |
| Q412R+V413M | 100 | 1530 | 1300 | 450 | 500 | 400 |
| Q412R | 60 | 2090 | 220 | 450 | 1150 | 910 |
| V413M | 120 | 2330 | 1250 | 650 | 1740 | 2340 |
a
Activity was measured as described in Experimental Procedures, and is expressed in Miller units.
b
narX allele located on plasmid pVJS1241 or pVJS2474 derivative.
c
Strain VJS5054 [Φ(narG-lacZ) ΔnarX narQ::Tn10].
d
Strain VJS5721 [Φ(napF-lacZ) Δ(narXL) narQ::Tn10].