Table 4.
Effects of narQ alterations on NarL- and NarP-dependent induction.
| Alleleb | β-Galactosidase sp acta |
|||
|---|---|---|---|---|
| NarL-dependent induction byc |
NarP-dependent induction byd |
|||
| None | Nitrate | None | Nitrate | |
| pHG165 | 5 | 5 | 100 | 90 |
| narQ+ | 50 | 1220 | 220 | 3140 |
| R381E | 110 | 1120 | 260 | 2780 |
| R381V | 150 | 1340 | 360 | 3060 |
| I382M | 120 | 1230 | 500 | 2770 |
| I382G | 360 | 1050 | 3350 | 2450 |
| I382S | 390 | 1140 | 2860 | 2570 |
| Y413R | 5 | 7 | 70 | 210 |
| F423Y | 290 | 1030 | 3160 | 2400 |
a
Activity was measured as described in Experimental Procedures, and is expressed in Miller units.
b
narQ alleles located on plasmid pVJS1231 derivative.
d
Strain VJS5054 [Φ(narG-lacZ) ΔnarX narQ::Tn10].
e
Strain VJS5721 [Φ(napF-lacZ) Δ(narXL) narQ::Tn10].