Table 5.
Oligonucleotides.
| Oligonucleotide | Sequencea | Purpose |
|---|---|---|
| ØHYL2726 | 5′–CATGAAGATGCGGGTGAGTTGTT | NarX(V413) revertant (coding strand) |
| ØHYL2727 | 5′–AACAACTCACCCGCATCTTCATG | NarX(V413) revertant (template strand) |
| ØHYL2728 | 5′–TGAAGATGCAGATGAGTTGTTTA | NarX(Q412) revertant (coding strand) |
| ØHYL2729 | 5′–TAAACAACTCATCTGCATCTTCA | NarX(Q412) revertant (template strand) |
| ØJSnarL-1 | 5′–GCAAAGGATCCAATCAGGAACCGGCTACTATC | His6-NarL (coding strand) |
| ØJSnarL-2 | 5′–CGCATCATCGAAGCTTTGGGAACCGTAATCAGA | His6-NarL (template strand) |
| ØJSnarP-1 | 5′–CAGCGGATCCCTGAAGCAACACCTTT | His6-NarP (coding strand) |
| ØJSnarP-2 | 5′–ATCGTCGGCAAGCTTCGGGCTATTTTTATT | His6-NarP (template strand) |
| ØLLC2560 | 5′–GCTCTAGATACCGTTCGCTGGAAGCGTCAGTAG | MBP-NarQ226 (coding strand) |
| ØLLC2562 | 5′–CCCAAGCTTCTACATTAACTGACTTTCCTCACCCTC | MBP-NarQ226 (template strand) |
| ØLLC2565 | 5′–GCTCTAGATATGCCGTACTTGAGCAGCGGGTTCAGG | MBP-NarX227 (coding strand) |
| ØLLC2567 | 5′–CCCAAGCTTCTACTCATGGGTATCTCC | MBP-NarX227 (template strand) |
| ØLLC2980 | 5′–CAGGTACTTTCTTACTTAGAGATCCAGTTGACGTTACTGAAGCG | NarQ(R381E) substitution (coding strand) |
| ØLLC2981 | 5′–GTAACGTCAACTGGATCTCTAAGTAAGAAAGTACCTGAGC | NarQ(R381E) substitution (template strand) |
| ØLLC2982 | 5′–GTTGAATGATGCTCGCCGGCAGTTACGC | NarQ(Y413R) substitution (coding strand) |
| ØLLC2983 | 5′–GCGTAACTGCCGGCGAGCATCATTCAAC | NarQ(Y413R) substitution (template strand) |
| ØLLC2984 | 5′–GCTGTTGACTACTTACCGCCTGACGCTG | NarQ(F423Y) substitution (coding strand) |
| ØLLC2985 | 5′–CAGCGTCAGGCGGTAAGTAGTCAACAGC | NarQ(F423Y) substitution (template strand) |
| ØLLC2992 | 5′–CTGGCTCAGGTACTTTCTTACTTANNNATCCAGTTGACGTTACTGAAG | NarQ(R381) randomization (coding strand) |
| ØLLC2993 | 5′–GAAAGTACCTGAGCCAGCGAGTC | NarQ(R381 & I382) randomization (template strand) |
| ØLLC2994 | 5′–CAGGTACTTTCTTACTTACGTNNNCAGTTGACGTTACTGAAGCGTTC | NarQ(I382) randomization (coding strand) |
a
Underlined sequence denotes introduced restriction endonuclease sites or codon substutions.