TABLE 2.
Reactions of anti-HA MAbs with escape mutants of Sw/HK/9/98-MA in the HI assay and ELISA
| MAb | Reactiona with escape mutant:
|
||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| m15F1 | Sw/HK/9/98-MP | mG9-27 | m18G4 | mG1-29 | m19A10 | m18B1 | m7B10 | m3D11 | m8C4 | m18B10 | m7B10-18G4 | m7B10-18G4′ | m18G4-18B1 | mG1-29-18G4 | m15F1-3D11 | m3D11-18G4 | m15F1-18B10 | m7B10-18B10 | |
| 15F1 | − | + | + | + | + | + | + | + | + | − | − | + | + | + | + | − | + | − | + |
| G9-27 | − | − | − | − | − | + | + | + | + | + | + | − | − | − | − | − | − | − | + |
| G9-6 | − | − | − | − | − | + | + | + | + | + | + | − | − | − | − | − | − | − | + |
| G1-26 | − | − | − | − | − | + | + | + | + | + | + | − | − | − | − | − | − | − | + |
| 18G4 | + | − | − | − | + | + | + | + | + | + | + | − | − | − | − | + | − | + | + |
| G9-25 | + | − | − | − | − | + | + | + | + | + | + | + | + | − | − | + | − | + | + |
| G1-29 | + | − | − | − | − | + | + | + | + | + | + | − | − | − | − | + | − | − | − |
| 19A10 | + | + | + | + | + | −* | − | + | + | + | + | + | + | − | + | + | + | + | + |
| 18B1 | + | + | + | + | + | − | − | − | − | + | + | − | − | − | + | − | − | − | − |
| 2F4 | + | + | + | + | + | − | − | − | − | + | + | − | − | − | + | − | − | − | − |
| 7B10 | + | + | + | + | + | −* | − | − | − | + | + | − | − | − | + | + | + | − | − |
| 3D11 | + | − | − | − | + | − | + | − | − | + | + | − | − | − | − | − | − | − | − |
| 4G3 | + | − | − | − | + | − | − | − | − | + | + | − | − | − | − | − | − | − | − |
| 8C4 | + | + | + | + | + | + | + | −* | −* | − | − | −* | −* | + | − | + | + | − | − |
| 18B10 | + | + | + | + | + | + | + | + | + | − | − | + | + | + | + | + | + | − | − |
a
+, HI titer similar to that obtained with wild-type virus (≤8-fold difference) and antibody-virus binding in ELISA >75%; −, HI titer at least 16 times less than that obtained with wild-type virus and binding in ELISA <25%; −*, HI titer at least 16 times less than that obtained with wild-type virus, but binding in ELISA >50%. Percent binding in ELISA was calculated from the equation A = 100 × (Bxv/Bpv), where A is the amount of antibody bound as a percentage of that binding to wild-type virus (100%), Bxv is binding of the MAb to the test virus, and Bpv is binding of pooled MAbs to the test virus.