TABLE 5.
Effect of amino acid changes in the HA of Sw/HK/9/98-MA virus on virulence in mice
| Virulence | Virus (amino acid change) | Virulencea (EID50/MLD50 ratio ± SE) |
|---|---|---|
| High | Sw/HK/9/98-MA | 4.01 ± 0.48 |
| m7B10 (N193D) | 3.64 ± 0.38 | |
| m18G4-18B1 (S133N, L226Q) | 3.73 ± 0.53 | |
| mG9-27 (S133N) | 3.96 ± 0.43 | |
| m3D11 (N193D) | 4.16 ± 0.08 | |
| m7B10-18G4′ (T135K, N193D) | 4.16 ± 0.44 | |
| m15F1-18B10 (N193T, P162L) | 4.28 ± 0.46 | |
| m18B1 (D145E) | 4.35 ± 0.16 | |
| m18G4 (S133N) | 4.36 ± 0.37 | |
| m15F1 (P162L) | 4.4 ± 0.47 | |
| m19A10 (L226Q) | 4.50 ± 0.25 | |
| m7B10-18G4 (S116I, T135K, N193D) | 4.57 ± 0.41 | |
| mG1-29 (K157T) | 4.58 ± 0.34 | |
| Moderate | mG1-29-18G4 (S133N, K157T) | 5.87 ± 0.29 |
| m3D11-18G4 (S133N, N193D) | 5.74 ± 0.44 | |
| Sw/HK/9/98-MP (S133N) | 5.64 ± 0.38 | |
| m15F1-3D11 (T135A, F147L, P162L) | 5.06 ± 0.59 | |
| m7B10-18B10 (N193D, T189A) | 4.95 ± 0.41 | |
| Low | m18B10 (T198N) | 7.06 ± 0.26 |
| m8C4 (T198N) | 7.20 ± 0.43 | |
| Sw/HK/9/98 | 6.74 ± 0.43 |
a
Expressed as log10 EID50/MLD50 ± standard error × tα,n−2, where tα is Student's coefficient with probability α (α = 0.95). Amino acids are numbered according to the HA of the H3 subtype.