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. 2004 Jan;78(1):158–167. doi: 10.1128/JVI.78.1.158-167.2004

FIG. 7.

FIG. 7.

Long-chain DNA synthesis by UL54 in the presence of UL44. Wild-type UL54 expressed in reticulocyte lysates was assayed for the ability to be stimulated by purified baculovirus-expressed UL44 by measuring the incorporation of labeled TTP on a poly(dA)-oligo(dT) template. The reaction products were visualized by autoradiography after electrophoresis on a 4% alkaline agarose gel. Lane 1 contains UL54 alone; lanes 2, 3, and 4 contain UL54 plus 1,000, 500, or 200 fmol of UL44, respectively; lane 5 contains 1,000 fmol of UL44 alone.