TABLE 1.
Parameters for binding of C-terminal UL54 peptide 1 to UL44a
| Protein | Mean value ± SDb
|
||||
|---|---|---|---|---|---|
| Stoichiometry | ΔH (kcal/mol) | Kd (μM) | ΔG (kcal/mol) | TΔS (kcal/mol) | |
| GST-UL44 | 1.1 ± 0.1 | −16.4 ± 2.1 | 0.67 ± 0.18 | −8.3 ± 1.2 | −8.1 ± 0.9 |
| GST-UL44ΔC290 | 1.0 ± 0.1 | −16.5 ± 1.9 | 0.52 ± 0.11 | −8.6 ± 0.9 | −7.9 ± 0.8 |
| UL44ΔC290 | 1.0 ± 0.1 | −15.3 ± 2.2 | 0.62 ± 0.09 | −8.4 ± 1.3 | −6.9 ± 1.1 |
| GST | -c | - | - | - | - |
| MBP-UL42ΔC340 | - | - | - | - | - |
a
Titrations were performed with 10-μl peptide injections. Heats of dilution of both peptide and protein were subtracted from the raw data prior to analysis. Nonlinear least-squares analysis of the raw data was performed with the fitting algorithm provided with the calorimeter. Values are means of three to five determinations.
b
SD, standard deviation.
c
-, No binding was detected.