Skip to main content
. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Stroke. 2010 Oct;41(10 Suppl):S64–S71. doi: 10.1161/STROKEAHA.110.595298

Figure 5. Notch-2 over-activation is associated with cell death in vitro.

Figure 5

(A) Western blot from hippocampal neuronal cultures shows that Notch-2 activation, (NICD2, arrow), occurs at 6 hours following OGD when activity, as measured by Arc/Arg 3.1, is elevated and when apoptosis, as measured by the presence of cleaved casp-3, is ongoing. (B) Immunohistochemistry with Notch-2 specific and cleaved casp-3 antibodies show that 6 hours following OGD the level of Notch-2 increases in culture and that the majority of the Notch-2 labeled cells also express cleaved casp-3, a hallmark of apoptosis. DAPI panels visualize condensed nuclei. (C) Immunostaining using a PLAP specific antibody shows that transfected neurons with GFP are also positive for PLAP, indicating the presence of pCLE and pCLEN2. (D) Cell-death scoring on transfected cells indicates that NICD2 over-expression by pCLEN2 is toxic under basal conditions (*: p< 0.05). Under OGD conditions there is an overall increase in cell-death and pCLE and pCLEN2 show similar cell-death scores. (All scale bars are 50μm).