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F420H2 cofactor binding to enzymes 3356 and 3380. The apparent KM's for F420H2 were calculated by measuring the velocity of the reaction with varying concentrations of F420H2 from 0.05 to 10 µM while maintaining all other components at 100 µM aflatoxin, 1 µM enzyme. For clarity the velocity is expressed as percent of maximum velocity. Empty circles, 3356; filled circles, 3380.
