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. Author manuscript; available in PMC: 2011 Feb 7.
Published in final edited form as: J Mol Biol. 2004 Feb 13;336(2):381–393. doi: 10.1016/j.jmb.2003.12.043

Figure 4.

Figure 4

The effect of τ on DnaB activity in vitro. (A) The helicase activity of DnaB as a function of ATP concentration. Arrows indicate annealed (substrate) and displaced (product) oligonucleotides. Lanes 1–14 are presented as percentage displacement of annealed oligo-nucleotides as a function of ATP concentration. Increasing concentrations of τ inhibit DnaB. The data are shown also in graphical representation. (B) The helicase activity of DnaB as a function of increasing τ to DnaB molar ratio (pentamer:hexamer). For comparison, a control experiment of the helicase activity of DnaB as a function of increasing DnaG:DnaB (monomer:monomer) molar ratio is shown. Lanes 1–6 are presented as percentage displacement of annealed oligonucleotides as a function of increasing τ to DnaB molar ratio. Increasing concentrations of DnaG stimulate DnaB. Lanes a and b in both panels represent annealed and boiled controls, respectively.