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. Author manuscript; available in PMC: 2011 Dec 22.
Published in final edited form as: Neuron. 2010 Dec 22;68(6):1082–1096. doi: 10.1016/j.neuron.2010.11.026

Figure 7.

Figure 7

Synaptic AMPA receptor complexes are modulated by both CNIH-2 and γ-8. Scaled representative traces of 200 ms (desensitization, A1) and 1 ms (deactivation, B1) glutamate-evoked responses onto recombinant cells expressing GluA1 alone or with γ-8, and / or CNIH-2 as indicated. (A2,B2) Calculated weighted tau of desensitization (A) or deactivation (B) fit with a bi-exponential function. (A3, B3) Calculated charge transfer from 200 ms (A) and 1 ms (B) glutamate application onto recombinant cells expressing GluA1 alone or with γ-8, and / or CNIH-2 as indicated. * denotes p<0.05 when compared to GluA1. # denotes p<0.05 when compared to γ-8. Representative traces (C1) and quantified resensitization (C2) and steady-state current (IGlu-SS) following glutamate application to wild type (+/+) or transfected stargazer (stg/stg) cerebellar granule neurons. Currents evoked from γ-8 transfectants show resensitization and this is prevented by co-transfection with CNIH-2. Note that CNIH-2 alone restored glutamate-evoked currents in 3 of 10 transfected neurons. (D) Synaptic AMPA receptor-mediated mEPSCs were recorded from cultured cerebellar granule neurons from wild type or stargazer mice transfected with CNIH-2 and / or γ-8. (D1) CNIH-2 synergizes with γ-8 to slow AMPA-receptor mEPSC decay constant (D2) without affecting mEPSC amplitude (D3) in transfected cerebellar granular neurons. Note the absence of synaptic AMPA receptor responses in stargazer neurons transfected with CNIH-2 alone (ND, not detectable). * denotes p<0.05 when compared to +/+. # denotes p<0.05 when compared to stg/stg + γ-8. Summary data are mean ± S.E.M. See also Figure S6.