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. 2004 Jan;78(1):399–412. doi: 10.1128/JVI.78.1.399-412.2004

FIG. 3.

FIG. 3.

Verification of UL34 mutant recombinant virus genomic structure by Southern blotting. (A) Structure 1, schematic representation of the HSV-1(F) genome expanded to show a portion of the UL32 ORF through a portion of the UL36 ORF. The probe used recognized the sequence between the indicated restriction sites. Structures 2 and 3, probe-specific fragments expected for SacI-digested HSV-1(F) and for vRR1205 #1 and vRR1205 #2 genomic DNA, respectively. Restriction site abbreviations: Nc, NcoI; Pm, PmlI; Sa, SacI. (B) Southern blot analysis of SacI-digested genomic DNA derived from HSV-1(F) (lane 1), vRR1205 #1 (lane 2), and vRR1205 #2 (lane 3). The DNA marker indicates sizes in kilobase pairs.