Figure 2.

Moderate caloric restriction induces changes in stress pathways. A, There were no significant changes in CRF gene expression levels in the CeA compared with controls. B, CRF levels in the BNST were significantly decreased after 3 weeks of caloric restriction and remained reduced after 1 week of refeeding with chow (Refed) (*p < 0.01). C, There were no changes in methylation of the CRF promoter at individual cytosines in the CeA. D, In the BNST, there were significant decreases in methylation at multiple cytosines in calorically restricted mice that remained down after 1 week chow refeeding (Refed) (*post hoc, p < 0.05) [ad libitum, n = 4; Rstr, n = 4; Rstr-Chow (Refed), n = 4]. In addition, there was an overall effect of caloric restriction on methylation (p < 0.05). Data are mean ± SEM.