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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: J Allergy Clin Immunol. 2010 Nov;126(5):1049–105910. doi: 10.1016/j.jaci.2010.07.023

Figure 1. Validation of Anti-TSLP Receptor Monoclonal Antibodies by Image-Based Flow Cytometry.

Figure 1

Activated THP-1 monocytes were stained for TSLPr, HLA-DR and DNA. (A) Dot plots representing gated focused single cells with DNA negative cells excluded. Boxes denote TSLPr+ gates determined by visual inspection of single cells inside the gate versus cells outside the gate. Green crosshairs denote single cells shown in panels B and C. (B) Single cell images of TSLPr+ cells using different anti-TSLPr clones. Images for each stain are overlayed on the brightfield image. (C) Composite images corresponding to cells shown in (B).